Imidazo[4′,5′:3,4]Benzo[1,2-<i>e</i>][1,4]Diazepins as New Heterocyclic Systems: Synthesis, Characterization and Their in Vitro Interactions with Benzodiazepine Receptors

In this work, some new heterocyclic systems 10-(aryl)-3-alkyl-6-methyl-6,8-dihydroimidazo[4′,5′:3,4]benzo[1,2-e][1, 4]diazepin-7(3H)-ones were obtained from the reaction of o-N-methylamino-ketone benzimidazoles with glycine in good yields. The structures compounds confirmed by elemental analyses, IR, mass, and NMR spectra. Also, their interactions studied vitro on recombinant benzodiazepine receptors (αxβ2/3γ2, x = 1–3, 5) expressed HEK293 cells. results revealed that displayed suitable affinity for α1β2 γ2 subtype (Ki 19–31 nM).